减数分裂
突触
生物
遗传学
染色体分离
拟南芥
同源重组
染色体
细胞生物学
计算生物学
基因
突变体
作者
Feng Chao,Elisabeth Roitinger,Otto Hudecz,María Cuacos,Jana Lorenz,Veit Schubert,Baicui Wang,Rui Wang,Karl Mechtler,Stefan Heckmann
出处
期刊:Nature plants
[Springer Nature]
日期:2023-03-13
卷期号:9 (4): 616-630
被引量:3
标识
DOI:10.1038/s41477-023-01371-7
摘要
During meiotic prophase I, sister chromatids are arranged in a loop-base array along a proteinaceous structure, called the meiotic chromosome axis. This structure is essential for synapsis and meiotic recombination progression and hence formation of genetically diverse gametes. Proteomic studies in plants aiming to unravel the composition and regulation of meiotic axes are constrained by limited meiotic cells embedded in floral organs. Here we report TurboID (TbID)-based proximity labelling (PL) in meiotic cells of Arabidopsis thaliana. TbID fusion to the two meiotic chromosome axis proteins ASY1 and ASY3 enabled the identification of their proximate 'interactomes' based on affinity purification coupled with mass spectrometry. We identified 39 ASY1 and/or ASY3 proximate candidates covering most known chromosome axis-related proteins. Functional studies of selected candidates demonstrate that not only known meiotic candidates but also new meiotic proteins were uncovered. Hence, TbID-based PL in meiotic cells enables the identification of chromosome axis proximate proteins in A. thaliana.
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