NMDA受体
奶油
药理学
抗焦虑药
慢性疼痛
基底外侧杏仁核
小胶质细胞
精神药理学
化学
神经科学
受体
医学
炎症
生物
内科学
生物化学
基因
转录因子
作者
Xin‐shang Wang,Shao-yu Guan,An Liu,Jiao Yue,Li‐ning Hu,Kun Zhang,Liu-kun Yang,Liang Lu,Zhen Tian,Ming Zhao,Shui-bing Liu
标识
DOI:10.1186/s13041-019-0453-4
摘要
Chronic pain is commonly accompanied with anxiety disorder, which complicates treatment. In this study, we investigated the analgesic and anxiolytic effects of Formononetin (FMNT), an active component of traditional Chinese medicine red clover (Trifolium pratense L.) that is capable of protecting neurons from N-methyl-D-aspartate (NMDA)-evoked excitotoxic injury, on mice suffering from complete Freund's adjuvant (CFA)-induced chronic inflammatory pain. The results show that FMNT administration significantly reduces anxiety-like behavior but does not affect the nociceptive threshold in CFA-injected mice. The treatment reverses the upregulation of NMDA, GluA1, and GABAA receptors, as well as PSD95 and CREB in the basolateral amygdala (BLA). The effects of FMNT on NMDA receptors and CREB binding protein (CBP) were further confirmed by the potential structure combination between these compounds, which was analyzed by in silico docking technology. FMNT also inhibits the activation of the NF-κB signaling pathway and microglia in the BLA of mice suffering from chronic inflammatory pain. Therefore, the anxiolytic effects of FMNT are partially due to the attenuation of inflammation and neuronal hyperexcitability through the inhibition of NMDA receptor and CBP in the BLA.
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