神经科学
下丘脑
电池类型
生物
细胞
原位杂交
视前区
计算生物学
基因
基因表达
遗传学
作者
Jeffrey R. Moffitt,Dhananjay Bambah-Mukku,Stephen W. Eichhorn,Eric M. Vaughn,Karthik Shekhar,Julio Pérez,Nimrod D. Rubinstein,Junjie Hao,Aviv Regev,Catherine Dulac,Xiaowei Zhuang
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2018-11-16
卷期号:362 (6416)
被引量:783
标识
DOI:10.1126/science.aau5324
摘要
The hypothalamus controls essential social behaviors and homeostatic functions. However, the cellular architecture of hypothalamic nuclei-including the molecular identity, spatial organization, and function of distinct cell types-is poorly understood. Here, we developed an imaging-based in situ cell-type identification and mapping method and combined it with single-cell RNA-sequencing to create a molecularly annotated and spatially resolved cell atlas of the mouse hypothalamic preoptic region. We profiled ~1 million cells, identified ~70 neuronal populations characterized by distinct neuromodulatory signatures and spatial organizations, and defined specific neuronal populations activated during social behaviors in male and female mice, providing a high-resolution framework for mechanistic investigation of behavior circuits. The approach described opens a new avenue for the construction of cell atlases in diverse tissues and organisms.
科研通智能强力驱动
Strongly Powered by AbleSci AI