黑腹果蝇
马尔皮基亚小管系统
生物
模式生物
果蝇属(亚属)
遗传模型
计算生物学
小管
细胞生物学
昆虫
肾
基因
遗传学
生态学
中肠
幼虫
作者
Shireen‐A. Davies,Pablo Cabrero,Richard Marley,Guillermo Martínez-Corrales,Saurav Ghimire,Anthony J. Dornan,Julian A. T. Dow
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 203-221
被引量:7
标识
DOI:10.1007/978-1-4939-9021-4_17
摘要
The insect renal (Malpighian) tubule has long been a model system for the study of fluid secretion and its neurohormonal control, as well as studies on ion transport mechanisms. To extend these studies beyond the boundaries of classical physiology, a molecular genetic approach together with the ‘omics technologies is required. To achieve this in any vertebrate transporting epithelium remains a daunting task, as the genetic tools available are still relatively unsophisticated. Drosophila melanogaster, however, is an outstanding model organism for molecular genetics. Here we describe a technique for fluid secretion assays in the D. melanogaster equivalent of the kidney nephron. The development of this first physiological assay for a Drosophila epithelium, allowing combined approaches of integrative physiology and functional genomics, has now provided biologists with an entirely new model system, the Drosophila Malpighian tubule, which is utilized in multiple fields as diverse as kidney disease research and development of new modes of pest insect control.
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