[Identification and Influence of Quorum Sensing on Anaerobic Ammonium Oxidation Process].

Anaerobic ammonium oxidation (ANAMMOX), an innovative nitrogen removal technology, has good prospects for applications. However, ANAMMOX bacteria grow slowly and are hardly accumulated in bioreactors. In this study, a UASB reactor inoculated with sludge from landfill leachate treatment plant was used for the start-up of ANAMMOX process. Besides, exogenous quorum sensing signals (DSF and AHL) were added to improve the adhesion of ANAMMOX sludge. The results showed that the UASB successfully started the ANAMMOX process within 150 days of operation. The total nitrogen removal rate reached 80% and the proportion of ANAMMOX bacteria rose to 20%. There was a low concentration of AHLs signal molecules in the ANAMMOX sludge. If the β-position substituent group of AHL added was a carbonyl group (including 3-oxo-C₆-HSL, 3-oxo-C₈-HSL, 3-oxo-C₁₀-HSL and 3-oxo-C₁₂-HSL), the adhesion growth ability of the ANAMMOX sludge could be improved. In the case of dosing with AHL molecules without β-position substituent groups, only C₆-HSL and C₁₂-HSL could promote the adhesion of ANAMMOX sludge. The additions of C₈-HSL, C₁₀-HSL and DSF all had negative effects on the adhesion of ANAMMOX sludge.