A novel fluorogenic probe for visualizing the hydrogen peroxide in Parkinson’s disease models

Parkinson’s disease (PD) is closely related to the oxidative stress induced by excess hydrogen peroxide (H 2 O 2 ) in organisms. Developing an efficient method for noninvasive and real-time H 2 O 2 detection is beneficial to investigate the role played by H 2 O 2 in PD. In this work, a novel fluorogenic probe (CBH) for living organisms H 2 O 2 detection has been designed, synthesized and characterized. The emission of CBH in PBS solution is very weak. However, when H 2 O 2 was added, the fluorescence of CBH solution was sharply increased for 12-fold, accompanied by the emission peak blue-shifted from 600 to 530 nm. Moreover, the response of CBH to H 2 O 2 is highly sensitive and selective and is not affected by various ROS/RNS, anions, cations, and amino acids. Based on the good performance of CBH for H 2 O 2 detection, it has been successfully applied to visualizing the H 2 O 2 concentration in living cells, Zebrafish and [Formula: see text]. elegans PD models.