Triterpene Glycosides from Sea Cucumber Holothuria leucospilota

AIM:To find new bioactive triterpene glycosides from the sea cucumber Holothuria leucospilota.METHODS:Column chromatography on silica gel and ODS RP-C18 and HPLC were used for the isolation and purification of triterpene glycosides,and their structures were elucidated on the basis of spectral data and chemical evidence.RESULTS:Seven triterpene glycosides were isolated from the sea cucumber H.leucospilota,and identified as leucospilotaside A(1),leucospilotaside B(2),leucospilotaside C(3),holothurin B(4),holothurin B2(5),echinoside B(6),and holothurin A(7).CONCLUSION:Compound 2 is a new triterpene glycoside,and compounds 1,3,5 and 6 have been isolated from this sea cucumber for the first time.