异质性
线粒体DNA
生物
假基因
计算生物学
DNA测序
DNA
基因组
核DNA
基因
遗传学
作者
Alexander G. Bury,Fiona Robertson,Angela Pyle,Brendan Payne,Gavin Hudson
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 433-447
被引量:3
标识
DOI:10.1007/978-1-0716-1270-5_27
摘要
In recent years, next-generation sequencing (NGS)Next-generation sequencing (NGS) has become a powerful tool for studying both inherited and somatic heteroplasmic mitochondrial DNA (mtDNA)Mitochondrial DNA (mtDNA) variation. NGS Next-generation sequencing (NGS) has proved particularly powerful when combined with single-cell isolation techniques, allowing the investigation of low-level heteroplasmic variants both between cells and within tissues. Nevertheless, there remain significant challenges, especially around the selective enrichment of mtDNA Mitochondrial DNA (mtDNA) from total cellular DNA and the avoidance of nuclear pseudogenes. This chapter summarizes the techniques needed to enrich, amplify, sequence, and analyse mtDNA Mitochondrial DNA (mtDNA) using NGS Next-generation sequencing (NGS) .
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