The Isolation and Deep Sequencing of Mitochondrial DNA

In recent years, next-generation sequencing (NGS)Next-generation sequencing (NGS) has become a powerful tool for studying both inherited and somatic heteroplasmic mitochondrial DNA (mtDNA)Mitochondrial DNA (mtDNA) variation. NGS Next-generation sequencing (NGS) has proved particularly powerful when combined with single-cell isolation techniques, allowing the investigation of low-level heteroplasmic variants both between cells and within tissues. Nevertheless, there remain significant challenges, especially around the selective enrichment of mtDNA Mitochondrial DNA (mtDNA) from total cellular DNA and the avoidance of nuclear pseudogenes. This chapter summarizes the techniques needed to enrich, amplify, sequence, and analyse mtDNA Mitochondrial DNA (mtDNA) using NGS Next-generation sequencing (NGS) .