鸡败血症支原体
生物
免疫筛选
插入(复合材料)
抗血清
限制酶
分子生物学
基因组文库
重组DNA
基因组DNA
分子克隆
千道尔顿
克隆(Java方法)
打开阅读框
核酸序列
抗原
支原体
病毒学
基因
肽序列
微生物学
限制性酶
遗传学
cDNA文库
工程类
机械工程
作者
Shuji Saito,Ayumi Fujisawa,Setsuko Ohkawa,Nobukazu Nishimura,Takaharu Abe,Kazumi Kodama,K. Kamogawa,S Aoyama,Yoshikazu Iritani,Yoshiyuki Hayashi
出处
期刊:Vaccine
[Elsevier]
日期:1993-01-01
卷期号:11 (10): 1061-1066
被引量:14
标识
DOI:10.1016/0264-410x(93)90134-j
摘要
A λgt11 clone, designated M1 and having a 0.8 kilobase (kb) insert, was selected by screening a Mycoplasma gallisepticum (M.g.) genomic DNA library with antisera against M.g. cells and their membrane proteins. The sequence of a 1.7 kb EcoR1 fragment of genomic DNA covering the entire M1 insert revealed a long open reading frame, TM-1, that encoded a polypeptide with a deduced molecular weight of 29 kDa. An antiserum raised in chicken against the TM-1 polypeptide, which was produced by recombinant Escherichia coli cells and purified by column chromatography, inhibited growth of M.g. cells in vitro. Moreover, chickens immunized with this polypeptide were partially protected from a challenge with virulent M.g. This polypeptide may serve as the basis for a vaccine against M.g. infection.
科研通智能强力驱动
Strongly Powered by AbleSci AI