Tetrazine–trans-cyclooctene ligation for the rapid construction of 18F labeled probes

A radiolabeling method for bioconjugation based on the Diels–Alder reaction between 3,6-diaryl-s-tetrazines and an 18F-labeled trans-cyclooctene is described. The reaction proceeds with exceptionally fast rates, making it an effective conjugation method within seconds at low micromolar concentrations.