细胞生物学
脂质双层融合
小泡
细胞内
生物
磷脂酰丝氨酸
快照25
细胞膜
膜蛋白
膜
生物化学
突触小泡
磷脂
作者
Chuanxi Cai,Haruko Masumiya,Noah Weisleder,Noriyuki Matsuda,Miyuki Nishi,Moonsun Hwang,Jae‐Woong Ko,Pei‐Hui Lin,Angela M. Thornton,Xiaoli Zhao,Zui Pan,Shinji Komazaki,Marco Brotto,Hiroshi Takeshima,Jianjie Ma
摘要
A phosphatidylserine-binding protein, MG53, is shown to participate in membrane repair. MG53 recruits vesicles to the repair site in an oxidation dependent manner and MG53-null mice develop progressive myopathy associated with defective membrane repair. Dynamic membrane repair and remodelling is an elemental process that maintains cell integrity and mediates efficient cellular function. Here we report that MG53, a muscle-specific tripartite motif family protein (TRIM72), is a component of the sarcolemmal membrane-repair machinery. MG53 interacts with phosphatidylserine to associate with intracellular vesicles that traffic to and fuse with sarcolemmal membranes. Mice null for MG53 show progressive myopathy and reduced exercise capability, associated with defective membrane-repair capacity. Injury of the sarcolemmal membrane leads to entry of the extracellular oxidative environment and MG53 oligomerization, resulting in recruitment of MG53-containing vesicles to the injury site. After vesicle translocation, entry of extracellular Ca2+ facilitates vesicle fusion to reseal the membrane. Our data indicate that intracellular vesicle translocation and Ca2+-dependent membrane fusion are distinct steps involved in the repair of membrane damage and that MG53 may initiate the assembly of the membrane repair machinery in an oxidation-dependent manner.
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