Based on the symbiotic and dissemination relationship of a copper-molybdenum ore in Tibet,developed a beneficial flotation flowsheet —first step was copper-molybdenum mixed flotation and then copper-molybdenum were separated. Copper-molybdenum mixed flotation used conventional flotation reagents. Copper-molybdenum separation used new reagent-- HX-1 for copper inhibitor. Experiments show that HX-1 was good to the copper-molybdenum separation. The content of copper was decreased to 0.03% in molybdenum concentrate.