小发夹RNA
小RNA
计算机科学
计算生物学
协议(科学)
克隆(Java方法)
简单(哲学)
表达式(计算机科学)
核糖核酸
生物
基因
遗传学
医学
程序设计语言
病理
哲学
替代医学
认识论
作者
Kenneth Chang,Krista Marran,Amy Valentine,Gregory J. Hannon
出处
期刊:CSH Protocols
[Cold Spring Harbor Laboratory]
日期:2013-07-01
卷期号:2013 (7): pdb.prot075853-pdb.prot075853
被引量:31
标识
DOI:10.1101/pdb.prot075853
摘要
Generating expression constructs for artificial microRNAs (miRNAs) is relatively straightforward. This protocol describes the creation of miR-30-based short hairpin RNA (shRNA) cassettes that are compatible with a number of standard vector systems. The principles outlined here can also be easily applied to other miRNA scaffolds or to simple snapback shRNAs. It is important to note that one must understand the processing of the artificial scaffold and be able to predict precisely the small RNAs that will be generated. Otherwise, no design principles can be effectively applied and the probability that any individual shRNA clone will work effectively will be greatly reduced.
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