Experimental study of digital microfluidic biochip

Digital microfluidic biochip was fabricated with a single-plate EWOD device. The EWOD microfluidic system consists of 1.4 mm wide, 10 mm long. An enzyme and DNA was driven by moving droplet through each electrode (dimension of 2 mm width and 2 mm long) that control by digital operation. The digital microfluidic biochip has been successfully designed fabricated and tested. The fundamental fluidic operation (transporting and merging) were studied by controlling the wetting property of the droplet surface through electric potential, which can be used for lab-on-a-chip or micro total analysis system (μTAS). The tested results confirm that ionized droplet can be transported with fully controlled manner by applying voltage less than 60 V. Moreover, the droplet can be merged by applied voltage to generate digital signal for two control electrodes at the same condition.