血管生成素受体
血管生成素
早产儿视网膜病变
免疫染色
血管内皮生长因子
病理
血管内皮生长因子A
共域化
新生血管
血管生成
胎盘生长因子
内界膜
免疫组织化学
医学
生物
癌症研究
眼科
细胞生物学
视网膜
血管内皮生长因子受体
胎龄
怀孕
遗传学
作者
Naoyasu Umeda,Hiroaki Ozaki,Hideyuki Hayashi,Hiroko Miyajima–Uchida,Kenji Oshima
出处
期刊:Ophthalmic Research
[S. Karger AG]
日期:2003-01-01
卷期号:35 (4): 217-223
被引量:34
摘要
The tyrosine kinase receptor Tie2 and its ligands, the angiopoietins (Angs), play important roles in vascular integrity and neovascularization, modulating vascular endothelial growth factor (VEGF) activity. To elucidate the potential role of Angs and the Tie2 system in retinopathy of prematurity (ROP), we have investigated the expression of Angs, Tie2 and VEGF within fibroproliferative membranes in ROP.Fibroproliferative membranes were obtained from 38 cases with stage 5 ROP at the time of vitrectomy. Membranes were fixed in formalin and embedded in paraffin. Each specimen was serially sectioned for immunohistochemistry. Polyclonal antibodies specific for Ang1, Ang2, Tie2 and VEGF were used for immunostaining. Immunoreactivity for von Willebrand factor (factor VIII) was also assessed to confirm the identity of vascular endothelial cells.Positive staining for Tie2 was observed in 23 of 38 specimens (60.5%). Tie2 was localized in vascularized regions of fibrovascular membranes and was co- expressed with VEGF and factor VIII. Ang2 stained positively in 18 of 38 (47.3%) serial sections where Tie2 was present, and was also co-expressed with VEGF and factor VIII. Ang1 was not generally observed in these specimens (3/38).VEGF and Ang2-Tie2 interactions may play an important role in the pathogenesis of ROP.
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