体内
高分子
生物物理学
化学
活力测定
程序性细胞死亡
细胞
受体
细胞凋亡
纳米技术
材料科学
生物化学
生物
生物技术
作者
Jun Wang,Jing Qi,Jian Fan,Yuchan You,Yan Du,Di Liu,Xiaoling Xu,Minjiang Chen,Shu Gao,Luwen Zhu,Xiaoying Ying,Jiansong Ji,Weishuo Li,Yong‐Zhong Du
出处
期刊:Nano Today
[Elsevier]
日期:2022-02-01
卷期号:42: 101360-101360
被引量:9
标识
DOI:10.1016/j.nantod.2021.101360
摘要
Drug-free macromolecular therapeutics induce cell apoptosis by clustering non-internalizing cell-surface receptors, which has demonstrated great promise in tumor therapy, particularly in terms of non-specific toxicities when compared with low-molecular-weight drugs. However, most reported drug-free macromolecular therapeutics involve a ‘two-step’ administration manner and the in vivo study is scarce, likely imposing difficulties on in vivo application. Here, we in-situ synthesized a drug-free macromolecular therapeutic in living system by near-infrared up-conversion controlled cross-linking. Taking CD20 receptor-positive B-cell lymphoma as the model disease, poly(2-hydroxyethyl methacrylate) with pendants of cinnamate groups (CA) were first introduced onto the surface of up-conversion nanoparticles (UCNP), which were then functionalized with anti-CD20 aptamer to give Apt-pHEMA(CA)@UCNP. After a local or systemic administration of Apt-pHEMA(CA)@UCNP plus the local application of 980-nm NIR laser, the growth of tumor was significantly suppressed with no detectable toxicities. Exploring the superior anti-tumor efficiency of Apt-pHEMA(CA)@UCNP combined with 980-nm NIR laser, we found that Apt-pHEMA(CA)@UCNP bound onto the surface of tumor cell by the interaction between CD20 receptors and anti-CD20 aptamers, without causing any reduction on cell viability. While after the local application of 980-nm laser, UCNP harvested the NIR light and converted it into UV light, which resulted in the cross-linking of CA groups, thus the clustering of CD20 receptors and cell apoptosis. We believe this NIR up-conversion controlled, in-situ synthesized drug-free macromolecular therapeutic expands the repertoire of macromolecular drugs and opens a new avenue for tumor therapy.
科研通智能强力驱动
Strongly Powered by AbleSci AI