交易激励
转录因子
基因
超氧化物歧化酶
脱落酸
生物
发起人
激活剂(遗传学)
细胞生物学
过氧化氢酶
基因表达
生物化学
氧化应激
作者
Xiaoxu Li,Qi Wang,Cun Guo,Jinhao Sun,Zhi-Yuan Li,Wang Yaofu,Aiguo Yang,Wen-xuan Pu,Yongfeng Guo,Junping Gao,Liu-Ying Wen
标识
DOI:10.3389/fpls.2022.817106
摘要
The NAC (NAM, ATAF1/2, and CUC2) family acts as one of the largest families of the transcription factor in the plant kingdom and was revealed to function as the important regulators in various environmental stresses. However, a few studies were reported about the biofunctions of the NAC transcription factor in tobacco. In the current study, we characterized a novel NAC transcription factor encoding the gene NtNAC053 in tobacco, which was significantly up-regulated when exposed to salt and drought treatments. The results of cis -acting elements analysis suggested that the promoter region of NtNAC053 gene possesses a number of stress-responsive elements, and this gene could be induced by exogenous abscisic acid (ABA) treatment. Moreover, the NtNAC053–GFP fusion protein was localized in the cell nucleus and possessed a transactivation domain in its C-terminal, implying that NtNAC053 may undertake as a transcriptional activator in tobacco. Notably, the overexpression of NtNAC053 in tobacco resulted in hypersensitivity to ABA treatment. Furthermore, these overexpression lines showed significantly enhanced tolerances to drought and salt stresses. Under salt and drought stresses, these overexpression lines possessed higher superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activities. Interestingly, the expressions of putative stress-related genes, including NtCOR15A , NtRAB18 , NtDREB1A , NtERF5 , NtKAT2 , and NtERD11 , were up-regulated in these overexpression lines when subjected to salt and drought stresses. The clues provided in our study suggested that the NtNAC053 gene encodes a novel NAC transcription factor and could confer the drought and salt stress tolerances by inspiring the downstream stress-responsive genes and antioxidant system in tobacco.
科研通智能强力驱动
Strongly Powered by AbleSci AI