循环肿瘤细胞
DNA甲基化
甲基化
肺癌
癌症研究
癌症
抗体
分子生物学
生物
病理
医学
免疫学
DNA
内科学
基因
基因表达
转移
生物化学
作者
Shuqiao Hu,Jiehuan Lin,Chaoyang Wu,Jun Ma,Shanshan Chen,Funan Chen
出处
期刊:Science of Advanced Materials
[American Scientific Publishers]
日期:2021-12-01
卷期号:13 (12): 2302-2312
被引量:4
标识
DOI:10.1166/sam.2021.4161
摘要
Through the dynamic detection of peripheral blood circulating tumor cells (CTCs) and the correlation analysis of DNA methylation, the application of relevant indicators of peripheral blood circulating tumor cells (CTCs) in patients with early lung cancer (LC). Double antibody (EpCAM and EGFR antibodies) modified nano-PLGA magnetic beads have been used for CTC sorting. Functional characterization and analysis were performed to confirm that the prepared nano-PLGA MB had good stability and specificity. Furthermore, the separation and identification of CTCs from lung cancer patients were realized by double-antibody nano-PLGA MB (Ep+ER nano-PLGA MB), suggesting that the system had high separation efficiency, with a positive rate of separation of >80%. Meanwhile, methylation-specific PCR was conducted following the extraction DNA from peripheral blood to analyze the methylation level of p16, MGMT and RASSF1A. Corresponding results revealed that the level of p16 methylation could be used as an important index for lung cancer screening. In addition, the association between CTC-DNA methylation and early screening of tumor was analyzed by integrating the clinical information and related indexes of patients. To sum up, in the screening and identification of lung cancer patients, auxiliary effect can be provided through dynamic monitoring of CTC count in peripheral blood and analysis of CTC-DNA methylation level. It is expected to provide scientific basis for screening, therapeutic strategy formulation, gene correlation analysis and prognosis monitoring of LC.
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