Incorporation material into medaka (Oryzias latipes) eggs by asymmetric burst pulses

The purpose of this study is to establish a simple method to incorporate materials into fertilized fish eggs by using some voltage pulses. Medaka (Oryzias latipes) eggs are used in our research. Medaka fish lay a lot of eggs every day, so it is easy to prepare samples for experiment. Furthermore we can observe their growth through transparent outer-shell with a microscope.