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Lifespan and ROS levels in different Drosophila melanogaster strains after 24 h hypoxia exposure

黑腹果蝇 生物 活性氧 长寿 氧化应激 黑腹菌 缺氧(环境) 遗传学 男科 野生型 生理学 突变体 毒理 氧气 基因 生物化学 医学 化学 有机化学
作者
Sandro Malacrida,Federica De Lazzari,Simona Mrakic‐Sposta,Alessandra Vezzoli,Mauro Agostino Zordan,Marco Bisaglia,Giulio Maria Menti,Nicola Meda,Giovanni Frighetto,Gerardo Bosco,Tomas Dal Cappello,Giacomo Strapazzon,Carlo Reggiani,M. Gussoni,Aram Megighian
出处
期刊:Biology Open [The Company of Biologists]
卷期号:11 (6) 被引量:6
标识
DOI:10.1242/bio.059386
摘要

During recent decades, model organisms such as Drosophila melanogaster have made it possible to study the effects of different environmental oxygen conditions on lifespan and oxidative stress. However, many studies have often yielded controversial results usually assigned to variations in Drosophila genetic background and differences in study design. In this study, we compared longevity and ROS levels in young, unmated males of three laboratory wild-type lines (Canton-S, Oregon-R and Berlin-K) and one mutant line (Sod1n1) as a positive control of redox imbalance, under both normoxic and hypoxic (2% oxygen for 24 h) conditions. Lifespan was used to detect the effects of hypoxic treatment and differences were analysed by means of Kaplan-Meier survival curves and log-rank tests. Electron paramagnetic resonance spectroscopy was used to measure ROS levels and analysis of variance was used to estimate the effects of hypoxic treatment and to assess ROS differences between strains. We observed that the genetic background is a relevant factor involved in D. melanogaster longevity and ROS levels. Indeed, as expected, in normoxia Sod1n1 are the shortest-lived, while the wild-type strains, despite a longer lifespan, show some differences, with the Canton-S line displaying the lowest mortality rate. After hypoxic stress these variances are amplified, with Berlin-K flies showing the highest mortality rate and most evident reduction of lifespan. Moreover, our analysis highlighted differential effects of hypoxia on redox balance/unbalance. Canton-S flies had the lowest increase of ROS level compared to all the other strains, confirming it to be the less sensitive to hypoxic stress. Sod1n1 flies displayed the highest ROS levels in normoxia and after hypoxia. These results should be used to further standardize future Drosophila research models designed to investigate genes and pathways that may be involved in lifespan and/or ROS, as well as comparative studies on specific mutant strains.
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