Sulfonamide-metabolizing microorganisms and mechanisms in antibiotic-contaminated wetland sediments revealed by stable isotope probing and metagenomics

基因组 稳定同位素探测 蛋白质细菌 微生物 生物 生物降解 环境化学 细菌 生物强化 微生物学 化学 生态学 基因 生物化学 遗传学 16S核糖体RNA
作者
Jianfei Chen,Yuyin Yang,Yanchu Ke,Xiuli Chen,Xinshu Jiang,Chao Chen,Shuguang Xie
出处
期刊:Environment International [Elsevier BV]
卷期号:165: 107332-107332 被引量:39
标识
DOI:10.1016/j.envint.2022.107332
摘要

Sulfonamide (SA) antibiotics are ubiquitous pollutants in livestock breeding and aquaculture wastewaters, which increases the propagation of antibiotic resistance genes. Microbes with the ability to degrade SA play important roles in SA dissipation, but their diversity and the degradation mechanism in the field remain unclear. In the present study, we employed DNA-stable isotope probing (SIP) combined with metagenomics to explore the active microorganisms and mechanisms of SA biodegradation in antibiotic-contaminated wetland sediments. DNA-SIP revealed various SA-assimilating bacteria dominated by members of Proteobacteria, such as Bradyrhizobium, Gemmatimonas, and unclassified Burkholderiaceae. Both sulfadiazine and sulfamethoxazole were dissipated mainly through the initial ipso-hydroxylation, and were driven by similar microbes. sadA gene, which encodes an NADH-dependent monooxygenase, was enriched in the 13C heavy DNA, confirming its catalytic capacity for the initial ipso-hydroxylation of SA in sediments. In addition, some genes encoding dioxygenases were also proposed to participate in SA hydroxylation and aromatic ring cleavage based on metagenomics analysis, which might play an important role in SA metabolism in the sediment ecosystem when Proteobacteria was the dominant active bacteria. Our work elucidates the ecological roles of uncultured microorganisms in their natural habitats and gives a deeper understanding of in-situ SA biodegradation mechanisms.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
Grondwet完成签到,获得积分10
刚刚
lee完成签到,获得积分10
刚刚
卡萨卡萨完成签到,获得积分10
1秒前
1秒前
liya发布了新的文献求助10
2秒前
yinxx发布了新的文献求助10
2秒前
viviCCC发布了新的文献求助30
2秒前
健忘芷珊发布了新的文献求助10
2秒前
骏驰天下完成签到,获得积分10
2秒前
320me666完成签到 ,获得积分10
2秒前
2秒前
2秒前
科研通AI5应助Soap采纳,获得10
3秒前
木心给沐秋的求助进行了留言
4秒前
4秒前
duohao2023完成签到,获得积分10
6秒前
深情安青应助健忘芷珊采纳,获得10
10秒前
10秒前
锖婧发布了新的文献求助10
10秒前
Alan关注了科研通微信公众号
10秒前
12秒前
灵儿完成签到,获得积分10
15秒前
15秒前
viviCCC完成签到,获得积分10
15秒前
15秒前
16秒前
16秒前
Alena完成签到,获得积分20
16秒前
16秒前
量子星尘发布了新的文献求助10
16秒前
CHENMILH完成签到,获得积分10
17秒前
18秒前
默默的紫真完成签到,获得积分10
18秒前
申申来啦发布了新的文献求助10
20秒前
11发布了新的文献求助10
20秒前
20秒前
21秒前
李健应助viviCCC采纳,获得30
21秒前
LALA发布了新的文献求助20
21秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 330
Composite Predicates in English 300
Aktuelle Entwicklungen in der linguistischen Forschung 300
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3983833
求助须知:如何正确求助?哪些是违规求助? 3527275
关于积分的说明 11235616
捐赠科研通 3265376
什么是DOI,文献DOI怎么找? 1802471
邀请新用户注册赠送积分活动 880533
科研通“疑难数据库(出版商)”最低求助积分说明 808135