Mobilisation of microbial indicators, microbial source tracking markers and pathogens after rainfall events

Climate change is expected to affect the Mediterranean region by causing an increase in the number of heavy rainfall events. The aim of this study was to assess the effect of extreme river flow variations due to rainfall on the persistence and mobilisation of various microorganisms. These included faecal pollution indicators (Escherichia coli (EC), somatic coliphages (SOMCPH) and sulphite reducing clostridia spores (SRC)), microbial source tracking indicators (Bacteroides thetaiotaomicron GA17 strain phages (GA17PH) and sorbitol fermenting bifidobacteria (SFBIF)), and two pathogens (Salmonella spp and Enterovirus). Water and sediment samples were taken at different distances from the river before and after heavy rainfall events. The microbial load was higher in sediment samples closer to the river course. The concentration of some faecal indicators (EC and SFBIF) increased in sediments and river water after rainfall events, whereas the most conservative parameter (SRC) showed almost no variation. After rainfall, the indicators persisted at a different rate. Salmonella spp and Enterovirus were detected in some samples but always at lower concentrations than the microbial indicators. In conclusion, sediments are reservoirs of faecal and MST indicators and pathogens and could therefore pose a risk of pathogen dissemination.