RNA聚合酶Ⅱ
生物
延伸系数
染色质免疫沉淀
转录因子II F
转录因子ⅡD
细胞生物学
转录因子ⅡE
抄写(语言学)
RNA聚合酶Ⅱ全酶
转录因子ⅡB
分子生物学
聚合酶
转录因子
RNA聚合酶
核糖核酸
转录调控
发起人
生物化学
酶
基因
基因表达
哲学
核糖体
语言学
作者
Michael Lidschreiber,Kristin Leike,Patrick Cramer
摘要
After transcription initiation, RNA polymerase (Pol) II escapes from the promoter and recruits elongation factors. The molecular basis for the initiation-elongation factor exchange during this transition remains poorly understood. Here, we used chromatin immunoprecipitation (ChIP) to elucidate the initiation-elongation transition of Pol II in the budding yeast Saccharomyces cerevisiae. We show that the early Pol II elongation factor Spt5 contributes to stable recruitment of the mRNA capping enzymes Cet1, Ceg1, and Abd1. Genome-wide occupancy for Cet1 and Ceg1 is restricted to the transcription start site (TSS), whereas occupancy for Abd1 peaks at ~110 nucleotides downstream, and occupancy for the cap-binding complex (CBC) rises subsequently. Abd1 and CBC are important for recruitment of the kinases Ctk1 and Bur1, which promote elongation and capping enzyme release. These results suggest that cap completion stimulates productive Pol II elongation.
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