IC50型
一氧化氮
肿瘤坏死因子α
脂多糖
巨噬细胞
前列腺素E2
药理学
传统医学
化学
生药学
一氧化氮合酶
前列腺素
前列腺素E
体外
生物
生物化学
生物活性
免疫学
医学
内分泌学
有机化学
作者
Supinya Tewtrakul,Sanan Subhadhirasakul,Pimpimon Tansakul,Sarot Cheenpracha,Chatchanok Karalai
摘要
The whole plant extract of Eclipta prostrata and its isolated compounds were tested for their antiinflammatory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin E2 (PGE2 ) and tumor necrosis factor-alpha (TNF-α) release in RAW264.7 cells, as well as for the antiinflammatory mechanism of the active compound on mRNA expression. Among the isolated compounds, orobol (5) exhibited the highest activity against NO release with an IC50 value of 4.6 μm, followed by compounds 1, 2 and 4 with IC50 values of 12.7, 14.9 and 19.1 μm, respectively. The IC50 value of compound 5 against PGE2 release was found to be 49.6 μm, whereas it was inactive towards TNF-α (IC50 > 100 μm). The mechanism of orobol (5) was found to down-regulate iNOS and COX-2 mRNA expression in a concentration-dependent manner. The present study may support the traditional use of Eclipta prostrata for the treatment of inflammatory-related diseases.
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