Microplastics meet invasive plants: Unraveling the ecological hazards to agroecosystems

The objective of this study was to assess the combined impact of environmental microplastic pollution and biological invasion which represent critical global eco-environmental challenges. The invasion of Solidago canadensis L. and soil microplastic contamination in the agroecosystem pose severe hazards to soil and plant ecology and human health. Oryza sativa L. (rice) was examined after individual and combined exposure to Solidago canadensis L. invasion (SI) and soil polyethylene microplastic contamination (MPc). Comparing the individual and combination treatments to the control, leaf biomass decreased, with varying changes in carbon, nitrogen, and phosphorus. Antioxidant enzyme activity and reactive oxygen species levels were significantly reduced following SI exposure and increased following the combined treatment (SI × MP). In contrast, ascorbate peroxidase and catalase activities were reduced after the combined treatment. Due to the confluence of various abiotic stressors, the combined treatment had a higher impact on leaf metabolites than the singular SI and MPc treatments. However, in comparison, the combined treatment significantly influenced the metabolic profile. In conclusion, the interaction between SI and MPc resulted in significant metabolic alterations. These changes were characterized by shifts in metabolite pools influenced by antioxidant enzyme activities and nutrient content, ultimately enhancing defense mechanisms within rice crops. Consequently, these stressors threaten the food safety, sustainability, and agricultural output of crops. The co-exposure of invasive plants and microplastics sheds light on the bio-ecological risks associated with microplastics in staple foods and offers valuable insights into the phytotoxicity of invasive plants in the presence of polyethylene microplastics.