ABSTRACTPurpose To evaluate and compare the results of dichoptic training in Argentinian children and adults with anisometropic amblyopia.Methods Prospective non-comparative study enrolling 41 subjects with anisometropic amblyopia (age, 6–60 years old). Two groups were differentiated according to age, children (6–16 years, 24 subjects) and adults (>17 years, 17 subjects). All patients were treated with the Bynocs® platform (Kanohi Eye Pvt. Ltd, India) following a protocol of 30 sessions of training of 30 min daily 5 times a week for 6 weeks. Changes in corrected distance visual acuity (CDVA) and binocular function (BF) score with treatment were analyzed.Results In the whole sample, CDVA in the amblyopic eye improved significantly, with a mean change of 0.30 logMAR (p < .001). Likewise, a significant improvement was also found in BF score (p < .001), with a mean change of 1.14 log units. The change achieved in CDVA was significantly correlated with the baseline CDVA in the amblyopic eye (r=-0.568, p < .001). Furthermore, no significant differences were found between age groups in the change achieved in CDVA (p = .431) and BF with therapy (p = .760).Conclusions Dichoptic training with the digital platform evaluated provides an effective improvement of visual acuity and binocular function in children and adults with anisometropic amblyopia.KEYWORDS: Anisometropic amblyopiabinocular functiondichoptic trainingstereopsisvisual acuity Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingDavid P. Piñero received funding from BYNOCS 2020 SL within the framework of the research project BYNOCS1-21A in collaboration with the University of Alicante.