Natural xylose-derived carbon dots towards efficient semi-artificial photosynthesis

Photosynthesis by plants stores sunlight into chemicals and drives CO2 fixation into sugars with low biomass conversion efficiency due to the unoptimized solar spectrum utilization and various chemical conversion possibilities that follow H2O oxidation. Expanding the solar spectrum utilization and optimizing the charge transfer pathway of photosynthesis is critical to improving the conversion efficiency. Here, a group of carbon dots (CDs) with distinct content of sp2 CC domain are prepared by one-step carbonization of natural xylose, which penetrated natural chloroplasts and integrated with the grana thylakoid to promote in vitro photosynthesis. Structural characterization and electrochemical results reveal the positive impact of graphitization degree on the electron transport capacity of CDs. Classic Hill reaction and ATP production demonstrate the enhanced photosynthetic activity resulting from the CDs-mediated electron transfer of photosystem II. In-depth studies of the structure-function relationship prove the synergistic effect of intensified biotic-abiotic interaction between CDs and chloroplast, lower charge transfer resistance, and extended light absorption. This work posts a promising method to optimize electron transport and improve natural photosynthesis using artificial interventions.