蜘蛛丝
材料科学
丝绸
自愈水凝胶
药物输送
组织工程
重组DNA
绿色荧光蛋白
生物物理学
纳米技术
细胞培养
脚手架
间充质干细胞
生物医学工程
细胞生物学
高分子化学
化学
复合材料
生物
生物化学
基因
遗传学
医学
作者
Tina Arndt,Urmimala Chatterjee,Olga Shilkova,Juanita Francis,Johan Lundkvist,Daniel Johansson,Benjamin Schmuck,Gabriele H. Greco,Agneta Nordberg,Yan Li,Lars U. Wahlberg,Maud Langton,Jan Johansson,Cecilia Götherström,Anna Rising
标识
DOI:10.1002/adfm.202303622
摘要
Abstract Hydrogels are useful drug release systems and tissue engineering scaffolds. However, synthetic hydrogels often require harsh gelation conditions and can contain toxic by‐products while naturally derived hydrogels can transmit pathogens and in general have poor mechanical properties. Thus, there is a need for a hydrogel that forms under ambient conditions, is non‐toxic, xeno‐free, and has good mechanical properties. A recombinant spider silk protein‐derived hydrogel that rapidly forms at 37 °C is recently developed. The temperature and gelation times are well‐suited for an injectable in situ polymerising hydrogel, as well as a 3D cell culture scaffold. Here, it is shown that the diffusion rate and the mechanical properties can be tuned by changing the protein concentration and that human fetal mesenchymal stem cells encapsulated in the hydrogels show high survival and viability. Furthermore, mixtures of recombinant spider silk proteins and green fluorescent protein (GFP) form gels from which functional GFP is gradually released, indicating that bioactive molecules are easily included in the gels, maintain activity and can diffuse through the gel. Interestingly, encapsulated ARPE‐19 cells are viable and continuously produce the growth factor progranulin, which is detected in the cell culture medium over the study period of 31 days.
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