Electrochemical Aptasensor for Selective and Sensitive Detection of Staphylococcus aureus Using Graphene oxide/poly Deep Eutectic Solvent/Nickel Oxide Nanocomposite Modified Electrode

Abstract Since that pathogenic bacteria are major threats to human health; this research describes the fabrication of an effective and sensitive platform based on graphene oxide/poly deep eutectic solvent/nickel oxide nano particles (GO/PDES/NiO NPs) nanocomposite at the surface of glassy carbon electrode for selective detection of Staphylococcus aureus (S. aureus) . The DNA aptamers were immobilized on the modified electrode through the EDC/NHS chemistry. Each aptasensor modification step was checked by with cyclic voltammetry (CV) and electrochemical impedance spectra (EIS) techniques. The linear dynamic range of the proposed electrochemical aptasensor covered a large variation of S. aureus concentrations from 10 1 – 10 8 CFU.mL −1 and a detection limit of 10 CFU.mL −1 under optimum conditions was obtained. Analysis of spiked tap water, lake water and human serum samples revealed high potential of this aptasensor as a sensitive, effective, and label‐free promising tool for S. aureus detection. This is the first time that poly deep eutectic solvents used in live bacteria detection.