电穿孔
嵌合抗原受体
转染
转导(生物物理学)
生物
细胞生物学
白细胞介素12
免疫学
免疫疗法
细胞培养
基因
细胞毒性T细胞
免疫系统
体外
遗传学
生物化学
作者
Zhicheng Du,Tianzhi Zhao,Xianjin Chen,Shijun Zha,Shu Wang
标识
DOI:10.1007/978-1-0716-3593-3_18
摘要
With the inherent antitumor function and unique “off-the-shelf” potential, genetically engineered human natural killer (NK) cells with chimeric antigen receptors (CARs) bear great promise for the treatment of multiple hematological malignancies and solid tumors. Current methods of producing large-scale CAR-NK cells mainly rely on mRNA transfection and viral vector transduction. However, mRNA CAR-NK cells were not stable in CAR expression while viral vector transduction mostly ended up with low efficiency. In this chapter, we described an optimized protocol to generate CAR-NK cells by using the piggyBac transposon system via electroporation and to further expand these engineered CAR-NK cells in a large scale together with artificial antigen-presenting feeder cells. This method can stably engineer human primary NK cells with high efficiency and supply sufficient scale of engineered CAR-NK cells for the future possible clinical applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI