结合珠蛋白
糖尿病性视网膜病变
糖基化
医学
疾病
视网膜病变
糖尿病
内科学
生物
内分泌学
遗传学
作者
Zhonghao Yuan,Zhizhen Lai,Yixin Zhang,Jiyun Zhang,Jinyu Zhou,Dan Li,Yu Wu,Jing Zhou,Zhili Li
标识
DOI:10.1002/prca.202300032
摘要
Abstract Purpose Diabetic retinopathy (DR), as one of the microvascular complications of diabetes, is a leading cause of acquired vision loss. Most DR cases are detected in the advanced stage through fundoscopy, making molecular biomarkers urgently needed for early diagnosis of DR. Experimental design Serum disease‐specific haptoglobin‐β (Hp‐β) chains of 100 patients with type 2 diabetes mellitus (T2DM) and 156 T2DM patients with non‐proliferative diabetic retinopathy (NPDR) were separated using polyacrylamide gel electrophoresis. After in‐gel digestion and enrichment, the intact N ‐glycopeptides were detected by mass spectrometry. Results Fucosylation of Hp‐β was significantly increased and sialylation of Hp‐β was significantly decreased in background DR (BDR, an early‐stage DR) patients compared with non‐diabetic retinopathy patients ( p < 0.05) and yielded area under curves (AUCs) of 0.801 and 0.829 in training and validation groups, respectively, which had an advantage over glycated hemoglobin A1c (AUC ≤ 0.691). Moreover, a significant increase in sialylated Hp‐β was found in severe NPDR patients compared with BDR patients and yielded an AUC of 0.828 to distinguish severe NPDR from BDR. Conclusion Changes in Hp‐β glycosylation are closely related to DR, and may be used for early diagnosis and screening of DR.
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