生物合成
加密
大肠杆菌
化学
量子点
合成生物学
纳米技术
酶
生物化学
基因
材料科学
计算生物学
生物
计算机科学
计算机安全
作者
Ling Niu,Yunfeng Lin,Chenyang Jin,Kai Jin,Zhen Liu,Tao Zhu,Xiaohui Zhu,Yong Zhang,Yihan Wu
标识
DOI:10.1002/ange.202315251
摘要
Abstract Microbial biosynthesis, as an alternative method for producing quantum dots (QDs), has gained attention because it can be conducted under mild and environmentally friendly conditions, distinguishing it from conventional chemical and physical synthesis approaches. However, there is currently no method to selectively control this biosynthesis process in a subset of microbes within a population using external stimuli. In this study, we have attained precise and selective control over the microbial biosynthesis of QDs through the utilization of an optogenetically engineered Escherichia coli ( E. coli ). The recombinant E. coli is designed to express smCSE enzyme, under the regulation of eLightOn system, which can be activated by blue light. The smCSE enzymes use L‐cysteine and Cd 2+ as substrates to form CdS QDs. This system enables light‐inducible bacterial biosynthesis of QDs in precise patterns within a hydrogel for information encryption. As the biosynthesis progresses, the optical characteristics of the QDs change, allowing living materials containing the recombinant E. coli to display time‐dependent patterns that self‐destruct after reading. Compared to static encryption using fluorescent QD inks, dynamic information encryption based on living materials offers enhanced security.
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