边缘地带
脾脏
细胞生物学
抗原
B细胞
转染
抗体
罗亚
B-1电池
生物
化学
红细胞
免疫学
分子生物学
T细胞
信号转导
抗原提呈细胞
免疫系统
细胞培养
遗传学
作者
Dan Liu,Benjamin Y. Winer,Marissa Chou,H.K. Tam,Ying Xu,Jinping An,James M. Gardner,Jason G. Cyster
标识
DOI:10.1038/s41590-023-01690-z
摘要
Spleen marginal zone (MZ) B cells are important for antibody responses against blood-borne antigens. The signals they use to detect exposure to blood are not well defined. Here, using intravital two-photon microscopy in mice, we observe transient contacts between MZ B cells and red blood cells that are in flow. We show that MZ B cells use adhesion G-protein-coupled receptor ADGRE5 (CD97) for retention in the spleen. CD97 function in MZ B cells depends on its ability to undergo autoproteolytic cleavage and signaling via Gα13 and ARHGEF1. Red blood cell expression of the CD97 ligand CD55 is required for MZ B cell homeostasis. Applying a pulling force on CD97-transfected cells using an optical C-trap and CD55+ beads leads to accumulation of active RhoA and membrane retraction. Finally, we show that CD97 deficiency leads to a reduced T cell-independent IgM response. Thus, our studies provide evidence that MZ B cells use mechanosensing to position in a manner that enhances antibody responses against blood-borne antigens.
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