多路复用
病毒学
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
唾液
灵敏度(控制系统)
传输(电信)
2019年冠状病毒病(COVID-19)
生物
计算机科学
医学
疾病
生物信息学
传染病(医学专业)
电信
生物化学
病理
电子工程
工程类
作者
Dohwan Lee,Tevhide Ozkaya‐Ahmadov,A. Fatih Sarioglu
出处
期刊:Small
[Wiley]
日期:2023-04-03
卷期号:19 (27)
被引量:2
标识
DOI:10.1002/smll.202208035
摘要
Abstract Severe acute respiratory syndrome‐coronavirus 2 (SARS‐CoV‐2) continues to threaten lives by evolving into new variants with greater transmissibility. Although lateral flow assays (LFAs) are widely used to self‐test for coronavirus disease 2019 (COVID‐19), these tests suffer from low sensitivity leading to a high rate of false negative results. In this work, a multiplexed lateral flow assay is reported for the detection of SARS‐CoV‐2 and influenza A and B viruses in human saliva with a built‐in chemical amplification of the colorimetric signal for enhanced sensitivity. To automate the amplification process, the paper‐based device is integrated with an imprinted flow controller, which coordinates the routing of different reagents and ensures their sequential and timely delivery to run an optimal amplification reaction. Using the assay, SARS‐CoV‐2 and influenza A and B viruses can be detected with ≈25x higher sensitivity than commercial LFAs, and the device can detect SARS‐CoV‐2‐positive patient saliva samples missed by commercial LFAs. The technology provides an effective and practical solution to enhance the performance of conventional LFAs and will enable sensitive self‐testing to prevent virus transmission and future outbreaks of new variants.
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