Qipengyuania spongiae sp. nov., isolated from marine sponge Cinachyrella kuekenthali

A novel bacterial strain, designated as PHS-Z21 T , was isolated from the marine sponge Cinachyrella kuekenthali collected from PG Dave’s Rock, Philippines. Cells of PHS-Z21 T are Gram-stain-negative, non-motile, pale-yellow-pigmented, short rods. PHS-Z21 T is able to grow at 10–40 ℃ (optimum, 30 ℃), pH 5.5–9.0 (optimum, pH 8.5) and with 3–9 % (w/v) NaCl (optimum, 4 %). Its 16S rRNA gene sequence shows 98.6 % similarity to Qipengyuania nanhaisediminis CGMCC 1.7715 T , 98.5 % similarity to Qipengyuania vulgaris 022-2-10 T and 98.4 % similarity to Qipengyuania flava SW-46 T , respectively. The phylogenetic tree based on 16S rRNA gene sequences reveals that PHS-Z21 T is clustered with Q. flava SW-46 T . The total genome of PHS-Z21 T is approximately 2 932 896 bp in size with a DNA G+C content of 64.7 %. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values among PHS-Z21 T and other type strains are 70.0–77.3 % (ANIb), 83.3–86.8 % (ANIm) and 13.0–26.9 % (dDDH), respectively. The dDDH and ANI values are below the standard cutoff criteria for delineating bacterial species. Percentage of conserved proteins (POCP) values between the genome of strain PHS-Z21 T and those of members of the genera Qipengyuania , Erythrobacter , Altererythrobacter and Alteriqipengyuania were 62.0–74.5 %, 55.8–63.2 %, 60.7–66.9 % and 63.9–66.8%, respectively, while the AAI values were 68.4–74.3 %, 63.8–65.9 %, 66.3–68.3 % and 64.7–66.9%, respectively. The major fatty acids of PHS-Z21 T are composed of summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ), C 18 : 1 ω7 c 11-methyl, C 16 : 0 and summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ). The polar lipids of PHS-Z21 T mainly consist of diphosphatidylglycerol, glycolipid, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and glycophospholipid. The respiratory lipoquinone was identified as Q-10. On the basis of the phenotypic and phylogenetic data, strain PHS-Z21 T represents a novel species of the genus Qipengyuania , for which the name Qipengyuania spongiae sp. nov. is proposed. The type strain is PHS-Z21 T (=MCCC 1K07849 T =KCTC 92590 T ).