A microfluidic chip capable of generating and trapping emulsion droplets for digital loop-mediated isothermal amplification analysis

A microfluidic droplet array chip was designed to execute the digital LAMP. This novel device was capable of 1) creating emulsion droplets, 2) sorting them into a trapping array, and 3) executing LAMP for only 40 min. Nucleic acids could be accurately quantified and the limit of detection was only single DNA molecule.