Liquid Chromatography-Mass Spectrometry Technique-A Review

Liquid Chromatography/Mass Spectrometry (LC/MS) is quickly becoming the preferred tool of liquid chromatographers. It is a powerful analytical technique that agglutinate the resolving powerful of liquid chromatography with the detection specificities of mass spectrometry. Liquid chromatography-mass spectrometry (LC-MS) is now became routine technique with the advancement of electro spray ionisation (ESI) provide a simple and robust interface. Since the newly developed API-based methods produce mild ionization, for structural elucidation studies it can be complemented by invoking fragmentation-induced collisions in the interface itself or by recourse to LC-tandem MS as realized with the help of a triple quadrupole system. It is applicable for of biological molecules and the use of tandem MS and stable isotope internal standards allows highly sensitive and accurate methods to be enlarge through some method optimisation to minimise ion repression effects. Method validation is of important between the process of drug discovery and development. The LC/MS data may be used to provide information about the molecular weight, structure, identity and quantity of particular sample components.