Wnt信号通路
离体
蛋氨酸
体内
地穴
碳酸钙-2
干细胞
连环素
信号转导
空肠
肠粘膜
细胞生物学
化学
肠上皮
生物
药理学
内科学
生物化学
上皮
体外
内分泌学
医学
生物技术
氨基酸
遗传学
作者
Jiayi Zhou,Zhe Wang,Sai-wu Zhang,Hualin Lin,Chun‐qi Gao,Jiangchao Zhao,Chengbo Yang,Xiuqi Wang
标识
DOI:10.1021/acs.jafc.9b04442
摘要
The intestinal epithelium is derived from intestinal stem cells (ISCs) and has direct contact with nutrients and toxins. However, whether methionine (Met) or a methionine hydroxyl analogue (2-hydroxy-4-(methylthio)butanoic acid (HMB)) can alleviate deoxynivalenol (DON)-induced intestinal injury remains unknown. Mice were treated orally with Met or HMB on days 1-11 and with DON on days 4-8. On day 12, the mice were sacrificed, and the jejunum was collected for crypt isolation and culture. Mouse enteroids were treated with DON and Met or HMB ex vivo. The results showed that Met and HMB increased the average daily feed intake and average daily gain of the mice. Met and HMB also improved the jejunal structure and barrier integrity and promoted ISC expansion, as indicated by the increased enteroid formation efficiency and area, under DON-induced injury conditions. In addition, DON-induced decreases in ISC activity were rescued Wnt/β-catenin signaling reactivation by Met or HMB in vivo and ex vivo. Collectively, our findings reveal that Met and HMB alleviated DON-induced intestinal injury by improving ISC expansion and reactivating Wnt/β-catenin signaling. Our study thus provides a nutritional intervention for intestinal diseases involving Wnt/β-catenin signaling.
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