化学
亚硝酸盐
循环伏安法
电化学气体传感器
傅里叶变换红外光谱
检出限
微分脉冲伏安法
胶体金
分子印迹聚合物
介电谱
分析化学(期刊)
核化学
纳米颗粒
电化学
电极
化学工程
硝酸盐
色谱法
纳米技术
选择性
材料科学
有机化学
物理化学
催化作用
工程类
作者
Alassane Diouf,Nezha El Bari,Benachir Bouchikhi
出处
期刊:Talanta
[Elsevier]
日期:2020-03-01
卷期号:209: 120577-120577
被引量:35
标识
DOI:10.1016/j.talanta.2019.120577
摘要
Human Exhaled Breath Condensate (EBC) contains markers of several inflammatory diseases. Its analysis is of interest to a number of researchers. Nitrite ions (NO2-), which are widely used in our daily lives, are nevertheless among these indicators. In this study, a simple, fast, portable, non-invasive and cheap electrochemical sensor is developed for the analysis of the nitrite profile in EBC. In this regard, sodium nitrite (NaNO2) was first immobilized on self-assembled 2-aminothiophenol (2-ATP) on a screen-printed gold electrode (Au-SPE). Then, a polymer matrix composed of polyvinyl alcohol (PVA) crosslinked with glutaraldehyde (GA) was combined with gold nanoparticles (Au-NPs) to cover the modified Au-SPE and complete the fabrication of the Ion Imprinted Polymer (IIP) sensor. The electrochemical behaviour of the sensor was monitored using Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Differential Pulse Voltammetry (DPV) methods, while the morphology and chemical composition of its layers were observed by infrared Fourier transform (FTIR), Atomic Force Microscopy (AFM) and Scanning Electron Microscopy coupled with energy dispersion X-Ray spectroscopy (SEM-EDS) techniques. In addition, after a successful control test using a Non-Imprinted Ion Polymer (NIIP) sensor, the obtained results demonstrated satisfactory sensitivity and selectivity to nitrite compared to co-existing interfering substances in EBC, such as nitrate, acetate and ammonium nitrate. Under improved experimental conditions, the nitrite IIP sensor exhibits responses proportional to nitrite concentrations (R2 = 0.96) over a concentration range of 0.5–50 μg mL−1 with a detection limit (LOD) of 4 μmol L−1 (signal-to-noise ratio S/N = 3). The proposed approach was well applied for the nitrite determination in EBC samples with a relative standard deviation (RSD = 4%) and could open clinical applications in respiratory medicine.
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