鼠李糖乳杆菌
炎症
碳酸钙-2
势垒函数
化学
紧密连接
益生菌
封堵器
上皮
促炎细胞因子
植物乳杆菌
结肠炎
杯状细胞
小肠
乳酸菌
肠道菌群
肠细胞
细胞凋亡
粘蛋白
作者
Lu Xiao,Shuang Xie,Lulu Ye,Linda Zhu,Qinghua Yu
标识
DOI:10.1002/mnfr.201900655
摘要
Scope The influence of the intestinal microbiota, such as Lactobacillus , on the intestinal mucosa, particularly intestinal stem cells, remains incompletely understood. In this study, mice and intestinal organoids are used to explore the regulatory effect of Lactobacillus on the proliferation and differentiation of intestinal epithelial cells. Methods and results This study demonstrates that S. typhimurium causes intestinal epithelial damage and affected growth of intestinal organoids. S. typhimurium also colonizes the intestine and then causes pathological changes to the intestinal epithelium, intestinal inflammation, and even death. However, L. acidophilus alleviates damage to intestinal organoids, increases the survival ratio of mice infected with S. typhimurium , and reduces tumor necrosis factor‐α (TNF‐α) secretion. Moreover, L. acidophilus affects the differentiation of epithelial cells through inhibition of the excessive expansion of goblet cells and Paneth cells induced by S. typhimurium to avoid over‐exhaustion. Finally, it is also demonstrated that L. acidophilus ameliorates overactivation of Wnt/β‐catenin pathway by Salmonella , depending on the contact with toll‐like receptor 2 (TLR2), to affect the proliferation of the intestinal epithelium. Conclusions This study demonstrates that L. acidophilus protects the intestinal mucosa against S. typhimurium infection through not only the inhibition of pathogen invasion but also determination of the fate of the intestinal epithelium.
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