相互作用体
LIM域
PDZ域
锚蛋白重复序列
细胞质
细胞生物学
计算生物学
双杂交筛选
生物
功能(生物学)
化学
锌指
酵母
遗传学
基因
转录因子
作者
Wenhao Wang,Yaxin Chen,Ying Chang,Wei Sun
出处
期刊:Medical science monitor basic research
日期:2020-07-27
卷期号:26
被引量:5
标识
DOI:10.12659/msmbr.924421
摘要
BACKGROUND:LMO2 belongs to the LIM-Only group of LIM domain protein superfamily. It is ubiquitously expressed in different types of tissues and locates either in the nucleus or in the cytoplasm depending on the tissue type. Till now the unique function of LMO2 was considered to be serving as a bridging or blocking molecule that mediates extensive protein-protein interactions. However, the exactly biological features of LMO2 interactome as well as LMO2 function spectrum remain largely unclear. MATERIAL AND METHODS:In this study, yeast 2-hybrid assay was firstly performed using LMO2 as the bait and the characteristic of LMO2 protein interactome was analyzed according to the yeast 2-hybrid data and other relative biological information primarily using bioinformatic method. RESULTS:Our data indicated that LMO2 favored interacting with peptides containing β-sheet structure and having relatively unstable confirmation. Moreover, several LMO2 favored interacting domains were identified, including WD40 repeat, coiled-coil, Ankyrin repeat, Zinc finger, PDZ, and SH3, and functions of these domain-containing members were dramatically enriched in some types of cancers. CONCLUSIONS:Our results revealed a LMO2 favored protein-interaction pattern in both secondary structure and domain level, and concentrated LMO2 function in kinds of cytoplasmic metabolism pathways as well as multiple types of cancers.
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