Ascorbic acid enhances in vitro primordial germ cell-like cell differentiation from mouse ESCs

Building upon this finding, we established a highly efficient and reproducible protocol for in vitro PGCLC differentiation from mouse embryonic stem cells (mESCs) by modulating epigenetic regulation through AA. This system provides a valuable platform for dissecting the molecular mechanism and epigenetic reprograming during early germ cell development and potential therapeutic applications.