毛细管电色谱
环糊精
化学
色谱法
傅里叶变换红外光谱
毛细管电泳
分析物
毛细管作用
对映体
泮托拉唑
分析化学(期刊)
化学工程
材料科学
有机化学
奥美拉唑
内科学
工程类
复合材料
医学
作者
Lijuan Gu,Jin Guan,Ziwei Huang,Hongyi Huo,Shuang Shi,Dongxiang Zhang,Feng Yan
出处
期刊:Electrophoresis
[Wiley]
日期:2022-04-28
卷期号:43 (13-14): 1446-1454
被引量:10
标识
DOI:10.1002/elps.202200029
摘要
In this work, a new open-tubular capillary electrochromatography (OT-CEC) column was prepared using β-cyclodextrin covalent organic framework (β-CD COF) as a stationary phase. Polydopamine was used to assist fabrication of β-CD COF on an inner wall of a fused-silica capillary. The coating layer on the capillary was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Electroosmotic flow (EOF) was also studied to evaluate the variation of the inner wall of immobilized columns. Furthermore, the chiral separation effectiveness of the fabricated capillary column was evaluated by CEC using enantiomers of several related proton pump inhibitors as model analytes, including omeprazole, lansoprazole, pantoprazole and tenatoprazole. The effects of bonding time and concentration of β-CD COF, the type, concentration and pH of buffer, applied voltage were investigated to obtain satisfactory enantioselectivity. In the optimum conditions, the enantiomers of four analytes were resolved within 15 min with resolutions of 1.63-2.62. The relative standard deviation values for migration times and resolutions of the analytes representing intraday and interday were less than 6.75% and 4.24%, respectively. The results reveal that β-CD COF has great potential as chiral-stationary phases for enantioseparation in CEC.
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