材料科学
荧光
替加环素
荧光团
药代动力学
吸收(声学)
药理学
化学
医学
抗生素
生物化学
光学
物理
复合材料
作者
Rouying Cai,Chenfang Miao,Liang Zhang,Yi Zhou,Yuebin Liu,Zheng Chen,Wendi Han,Zhengjun Huang,Xin Zhou,Shaohuang Weng
标识
DOI:10.1016/j.snb.2022.131721
摘要
Tigecycline (TIGE) plays a significant role in the management of complex and serious bacterial infection. The monitoring of TIGE content in plasma and the further application in pharmacokinetic (PK) study is important for clinical medicine, however, the facile and effective strategy is limited. Herein, a sensitive, accurate and facile method for TIGE detection was developed by fluorescence with F, N codoping carbon dots (F, N-CDs) as probe. F, N-CDs exhibited stable maximum emission fluorescence at 500 nm and large Stokes shift of 135 nm. Such property help to weaken the possible self-absorption of biological system and the spectral overlap of fluorophore. Thus, using F, N-CDs as probe, the method exhibited accuracy and similarity of the analytical performance of TIGE detection in human plasma and rat plasma. The validated method was successfully applied to the PK research of TIGE in rat after intravenous injection at three dose levels of 1 μg/g, 2 μg/g, and 4 μg/g at different time-points. The obtained PK parameters of TIGE using this method were basically consistent with current clinical practice. The findings demonstrated the application ability of F, N-CDs for PK study of TIGE ascribed to the clinical need of simple operation, accuracy and rapidity.
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