cGAS‐STING mediates cytoplasmic mitochondrial‐DNA‐induced inflammatory signal transduction during accelerated senescence of pancreatic β‐cells induced by metabolic stress

Abstract Type 2 diabetes mellitus (T2DM) is an age‐related disease characterized by impaired pancreatic β cell function and insulin resistance. Recent studies have shown that the accumulation of senescent β cells under metabolic stress conditions leads to the progression of T2DM, while senolysis can improve the prognosis. However, the specific mechanism of β cell senescence is still unclear. In this study, we found that the increased load of senescence pancreatic β cells in both older mice and obese mice induced by high‐fat diet (HFD) (DIO mice) was accompanied by activation of the Cyclic GMP‐AMP synthase (cGAS) ‐ stimulator of interferon genes (STING) pathway and using cGAS or STING small interfering RNA or STING inhibitor C176 to downregulate this pathway reduced the senescence‐associated secretion profile (SASP) and senescence of Min6 cells treated with palmitic acid or hydrogen peroxide. C176 intervention in DIO mice also significantly reduced the inflammation and senescence of the islets, thereby protecting the function of pancreatic β cell and glucose metabolism. Our study further revealed that mitochondrial DNA (mtDNA) leakage under metabolic stress conditions was critical for the activation of the cGAS‐STING pathway, which can be reversed by the mtDNA depleting agent ethidium bromide. Consistently, mtDNA leakage was more severe in older mice and was accelerated by a chronic HFD. In conclusion, we demonstrate that cytoplasmic mtDNA activates the cGAS‐STING pathway to mediate SASP during the accelerated senescence of pancreatic β‐cells induced by metabolic stress, and this process can be downregulated by the STING inhibitor C176.