A Simple and Rapid Lc-Ms/Ms Method for Simultaneous Quantification of Paracetamol and Pamabrom in Human Plasma

A combination of paracetamol (PCM) and pamabrom (PAMA) is generally prescribed to relieve patients of back pain and menstrual pain. In this study, for the first time, liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been developed and validated for simultaneous quantification of PCM and PAMA from human plasma. Both analytes and internal standard (IS) diclofenac were processed through liquid-liquid extraction and separated on a Kromasil C 18 (100 x 4.6 mm, 5μm) column with a simple optimized mobile phase. Quantification was performed on a triple quadrupole mass spectrometer employing electrospray ionization technique and was operated on multiple reaction monitoring (MRM) mode. The total chromatographic run time was 4 min with 0.4 mL/min flow rate. The calibration curve was linear ( r >0.995) over the range of 40–8000 ng/mL and 20–4000 ng/mL for PCM and PAMA, respectively. This method led to robust results, as it was validated for specificity, recovery, linearity, matrix effect, accuracy, precision, stability studies, and results were found within the acceptable criteria. Findings of the present study revealed that simplicity, rapidness, and sensitivity of this method offers scope for routine use in investigational or clinical monitoring of both drugs in human plasma.