片段(逻辑)
阿尔法(金融)
载体(分子生物学)
化学
药理学
计算生物学
医学
生物
计算机科学
重组DNA
生物化学
基因
算法
结构效度
护理部
患者满意度
作者
Г. А. Посыпанова,Владимир Александрович Макаров,Mariya V. Savvateeva,A. Bereznikova,E. S. Severin
标识
DOI:10.3109/1061186x.2013.765441
摘要
The alpha-fetoprotein (AFP) binding protein, a putative AFP receptor, is a tumour marker that is present on the surfaces of malignant cells. AFP enters cells through receptor-mediated endocytosis. The recombinant C-terminal fragment of AFP (AFP-3BC, which consists of amino acid residues 473-596) was obtained by the expression in Escherichia coli. AFP-3BC was shown to be bound specifically to the AFP putative receptor on tumour cells and accumulated by endocytosis in these cells in a similar manner to that of full-length human AFP. In lymphocytes, the binding and endocytosis of AFP-3BC were absent. Thus, the AFP receptor binding site was shown experimentally to be located within the AFP-3BC sequence. A conjugate of synthesised AFP-3BC with the antitumour antibiotic doxorubicin (DOX-AFP-3BC) demonstrated high antitumour activity in vitro. Thus, AFP-3BC can be used successfully as a vector for the targeted selective delivery of drugs into tumour cells.
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