A protocol for TILLING and eco-TILLING.

This chapter provides a brief overview of TILLING (Targeting Induced Local Lesion in Genomes; a novel, reverse genetics approach that combines the advantages of point mutations provided by chemical mutagenesis with the advantages of PCR-based mutational screening) and Eco-TILLING [a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis] procedures, and the potential applications of both techniques in plants. Detailed descriptions of the conduct of TILLING and Eco-TILLING are provided.