Production of β‐Alanine from Fumaric Acid Using a Dual‐Enzyme Cascade

Abstract The aim of this study was to develop an environmentally safe and efficient method for β‐alanine production using a dual‐enzyme cascade route with L‐aspartase (AspA) from E. coli and L‐aspartate‐α‐decarboxylase (PanD) from Corynebacterium glutamicum . Poor cooperativity in this system due to the divergent catalysis efficiencies of AspA and PanD led to an imbalance between the two reactions. To address this issue, we employed ribosome binding site regulation and gene duplication to coordinate the expression levels of AspA and PanD. Finally, we achieved β‐alanine production of 80.4±1.6 g L −1 with a conversion rate of 95.3±1.6 % in a 5‐L bioreactor. The dual‐enzyme cascade reported herein represents a promising strategy to meet industrial requirements for large‐scale β‐alanine production in the future.