Progress on Subcellular Proteomics

Subcellular proteomics is a new field in proteomics, the proteome of important subcellular structures has been analyzed by many strategies and techniques. Recently,almost all of subcellular structures have been analyzed by proteomic methods and proteomic analysis of sub-organelles or protein complexes have been developed.Moreover,to study proteome dynamics on some physiological or pathological conditions,quantitative analysis or differential analysis has become new direction of subcellular proteome.However,the challenge of subcellular proteome is how to explain the localization of the identified proteins,which are contaminated or really localized in this structure?This problem is a difficult and inevitable topic of subcellular proteome.The recent progress on subcellular proteomics, the challenge and prospect of subcellular proteomics are discussed.